FL-1211 (Vector Laboratories)
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Vector Laboratories
FL-1211
Fl 1211, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/FL-1211-2/Fluorescein+labeled+Griffonia+(Bandeiraea)+Simplicifolia+Lectin+II+(GSL+II%2C+BSL+II)/pmc12319253-22-0-5
Average 93 stars, based on 38 article reviews
Fl 1211, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/FL-1211-2/Fluorescein+labeled+Griffonia+(Bandeiraea)+Simplicifolia+Lectin+II+(GSL+II%2C+BSL+II)/pmc12319253-22-0-5
Average 93 stars, based on 38 article reviews
FL-1211 - by Bioz Stars,
2026-11
93/100 stars
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Transfection:Article Title: Multilevel plasticity and altered glycosylation drive aggressiveness in hypoxic and glucose-deprived bladder cancer cells. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] Abcam ab80039 Anti-B3GNT6 polyclonal antibody Invitrogen PA5-90159 Anti-C1GALT1 antibody Novus Biologicals NBP1-88477 Anti-C1GALT1C1 antibody Sigma-Aldrich HPA015632 Anti-GCNT1 antibody Abcam ab102665 Anti-ST3Gal I antibody Abcam ab96129 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific A-11012 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Invitrogen 65-6120 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific 31460 Monoclonal Mouse Anti-Human Ki-67 [MIB-1] Agilent M724029-2 Mouse IgG1 [MOPC-21] isotype control Abcam ab18443 Mouse monoclonal [CA19-9-203] to CA19-9 Abcam ab116024 Mouse monoclonal anti-tag-72 antibody [B72.3 + CC49] Abcam ab199002 Peroxidase AffiniPure Goat anti-Mouse IgG (H+L) Jackson ImmunoResearch 115-035-003 Polyclonal rabbit anti-mouse immunoglobulins/FITC DAKO F0313 Purified anti-human Sialyl Lewis X (dimeric), clone FH6 BioLegend 368102 Rabbit monoclonal [EP1215Y] to HIF-1 alpha Abcam ab51608 Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) Abcam ab23875 Chemicals, peptides, and recombinant proteins 0.05% Trypsin-EDTA Gibco 25300-062 4’,6-Diamidino-2-phenylindole dihydrochloride Thermo Fisher Scientific 62247 5,5’-dithiobis-(2-nitrobenzoic acid) Sigma-Aldrich 69-78-3 Accutase - Enzyme Cell Detachment Medium ThermoFisher Scientific 00-4555-56 Acetic acid Sigma-Aldrich A6283 Acetonitrile Merk 14261-2L Acetyl coenzyme A trisodium salt Sigma-Aldrich A2056 Ammonium acetate,BioXtra, R98% Sigma-Aldrich A7330 Antigen Unmasking Solution, Citrate-Based Vector Laboratories H-3300-250 Cisplatin 1mg/ml concentrate solution for infusion Accord Healthcare, S.L.U Crystal Violet Sigma-Aldrich 61135 Deferoxamine mesylate salt Sigma-Aldrich D9533 Dimethyl sulfoxide Sigma-Aldrich D26650 DL-Dithiothreitol Sigma-Aldrich D9779 Formaldehyde/Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, 7.4 pH 10 ML Electron Microscopy Sciences 15949 Formalin solution, neutral buffered, 10% Sigma-Aldrich HT501128 Formic acid Merk 5.33002.0050 GeneArtTM Platinum Cas9 Nuclease Thermo Fisher Scientific B25640 Glicerol for molecular biology, R99.0% Sigma-Aldrich G5516 Guanidine hydrochloride Sigma-Aldrich G4505 HaltTM Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific 78440 (Continued on next page) e1 iScience 28, 111758, February 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology Sigma-Aldrich H1758 jetPRIME transfection reagent PolyPlus Transfection 114-01 Lead citrate Electron Microscopy Sciences 22410 Magnesium chloride anhydrous Sigma-Aldrich M8266 Methanol Merck 34860-1L-R MTT (3-(4,5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) ThermoFisher Scientific M6494 Neuraminidase from Clostridium perfringens Sigma-Aldrich N5631 NONIDETTM P 40 Substitute Bioextra, mix Sigma-Aldrich 74385 Osmium tetroxide Electron Microscopy Sciences 20816-12-0 Oxaloacetic acid Sigma-Aldrich 328-42-7 Paraformaldehyde Sigma-Aldrich 158127 PNGase F Glycosidase from Elizabethkingia miricola Promega PROMV4831 Ponceau S anionic dye Sigma-Aldrich P3504 Potassium hydroxide Sigma-Aldrich 306568 Puromycin, Dihydrochloride EMD Millipore 540222 SeaPlaque Agarose Lonza 50101 Sodium borohydride Honeywell FlukaTM 7132 Sodium chloride Sigma-Aldrich 7647-14-5 TritonTM-X Sigma-Aldrich T8787 Trizma base Sigma-Aldrich T6066 Trypan Blue Stain (0.4%) Gibco 15250-061 UAR-EMS Uranyl Acetate Replacement Stain Electron Microscopy Science 22405 Biotinylated Peanut Agglutinin (PNA) Vector Laboratories B-1075-5 Biotinylated Vicia Villosa Lectin (VVA) Vector Laboratories B-1235 Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Electron Microscopy:Article Title: Multilevel plasticity and altered glycosylation drive aggressiveness in hypoxic and glucose-deprived bladder cancer cells. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] Abcam ab80039 Anti-B3GNT6 polyclonal antibody Invitrogen PA5-90159 Anti-C1GALT1 antibody Novus Biologicals NBP1-88477 Anti-C1GALT1C1 antibody Sigma-Aldrich HPA015632 Anti-GCNT1 antibody Abcam ab102665 Anti-ST3Gal I antibody Abcam ab96129 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific A-11012 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Invitrogen 65-6120 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific 31460 Monoclonal Mouse Anti-Human Ki-67 [MIB-1] Agilent M724029-2 Mouse IgG1 [MOPC-21] isotype control Abcam ab18443 Mouse monoclonal [CA19-9-203] to CA19-9 Abcam ab116024 Mouse monoclonal anti-tag-72 antibody [B72.3 + CC49] Abcam ab199002 Peroxidase AffiniPure Goat anti-Mouse IgG (H+L) Jackson ImmunoResearch 115-035-003 Polyclonal rabbit anti-mouse immunoglobulins/FITC DAKO F0313 Purified anti-human Sialyl Lewis X (dimeric), clone FH6 BioLegend 368102 Rabbit monoclonal [EP1215Y] to HIF-1 alpha Abcam ab51608 Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) Abcam ab23875 Chemicals, peptides, and recombinant proteins 0.05% Trypsin-EDTA Gibco 25300-062 4’,6-Diamidino-2-phenylindole dihydrochloride Thermo Fisher Scientific 62247 5,5’-dithiobis-(2-nitrobenzoic acid) Sigma-Aldrich 69-78-3 Accutase - Enzyme Cell Detachment Medium ThermoFisher Scientific 00-4555-56 Acetic acid Sigma-Aldrich A6283 Acetonitrile Merk 14261-2L Acetyl coenzyme A trisodium salt Sigma-Aldrich A2056 Ammonium acetate,BioXtra, R98% Sigma-Aldrich A7330 Antigen Unmasking Solution, Citrate-Based Vector Laboratories H-3300-250 Cisplatin 1mg/ml concentrate solution for infusion Accord Healthcare, S.L.U Crystal Violet Sigma-Aldrich 61135 Deferoxamine mesylate salt Sigma-Aldrich D9533 Dimethyl sulfoxide Sigma-Aldrich D26650 DL-Dithiothreitol Sigma-Aldrich D9779 Formaldehyde/Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, 7.4 pH 10 ML Electron Microscopy Sciences 15949 Formalin solution, neutral buffered, 10% Sigma-Aldrich HT501128 Formic acid Merk 5.33002.0050 GeneArtTM Platinum Cas9 Nuclease Thermo Fisher Scientific B25640 Glicerol for molecular biology, R99.0% Sigma-Aldrich G5516 Guanidine hydrochloride Sigma-Aldrich G4505 HaltTM Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific 78440 (Continued on next page) e1 iScience 28, 111758, February 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology Sigma-Aldrich H1758 jetPRIME transfection reagent PolyPlus Transfection 114-01 Lead citrate Electron Microscopy Sciences 22410 Magnesium chloride anhydrous Sigma-Aldrich M8266 Methanol Merck 34860-1L-R MTT (3-(4,5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) ThermoFisher Scientific M6494 Neuraminidase from Clostridium perfringens Sigma-Aldrich N5631 NONIDETTM P 40 Substitute Bioextra, mix Sigma-Aldrich 74385 Osmium tetroxide Electron Microscopy Sciences 20816-12-0 Oxaloacetic acid Sigma-Aldrich 328-42-7 Paraformaldehyde Sigma-Aldrich 158127 PNGase F Glycosidase from Elizabethkingia miricola Promega PROMV4831 Ponceau S anionic dye Sigma-Aldrich P3504 Potassium hydroxide Sigma-Aldrich 306568 Puromycin, Dihydrochloride EMD Millipore 540222 SeaPlaque Agarose Lonza 50101 Sodium borohydride Honeywell FlukaTM 7132 Sodium chloride Sigma-Aldrich 7647-14-5 TritonTM-X Sigma-Aldrich T8787 Trizma base Sigma-Aldrich T6066 Trypan Blue Stain (0.4%) Gibco 15250-061 UAR-EMS Uranyl Acetate Replacement Stain Electron Microscopy Science 22405 Biotinylated Peanut Agglutinin (PNA) Vector Laboratories B-1075-5 Biotinylated Vicia Villosa Lectin (VVA) Vector Laboratories B-1235 Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), MTT Assay:Article Title: Multilevel plasticity and altered glycosylation drive aggressiveness in hypoxic and glucose-deprived bladder cancer cells. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] Abcam ab80039 Anti-B3GNT6 polyclonal antibody Invitrogen PA5-90159 Anti-C1GALT1 antibody Novus Biologicals NBP1-88477 Anti-C1GALT1C1 antibody Sigma-Aldrich HPA015632 Anti-GCNT1 antibody Abcam ab102665 Anti-ST3Gal I antibody Abcam ab96129 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific A-11012 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Invitrogen 65-6120 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific 31460 Monoclonal Mouse Anti-Human Ki-67 [MIB-1] Agilent M724029-2 Mouse IgG1 [MOPC-21] isotype control Abcam ab18443 Mouse monoclonal [CA19-9-203] to CA19-9 Abcam ab116024 Mouse monoclonal anti-tag-72 antibody [B72.3 + CC49] Abcam ab199002 Peroxidase AffiniPure Goat anti-Mouse IgG (H+L) Jackson ImmunoResearch 115-035-003 Polyclonal rabbit anti-mouse immunoglobulins/FITC DAKO F0313 Purified anti-human Sialyl Lewis X (dimeric), clone FH6 BioLegend 368102 Rabbit monoclonal [EP1215Y] to HIF-1 alpha Abcam ab51608 Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) Abcam ab23875 Chemicals, peptides, and recombinant proteins 0.05% Trypsin-EDTA Gibco 25300-062 4’,6-Diamidino-2-phenylindole dihydrochloride Thermo Fisher Scientific 62247 5,5’-dithiobis-(2-nitrobenzoic acid) Sigma-Aldrich 69-78-3 Accutase - Enzyme Cell Detachment Medium ThermoFisher Scientific 00-4555-56 Acetic acid Sigma-Aldrich A6283 Acetonitrile Merk 14261-2L Acetyl coenzyme A trisodium salt Sigma-Aldrich A2056 Ammonium acetate,BioXtra, R98% Sigma-Aldrich A7330 Antigen Unmasking Solution, Citrate-Based Vector Laboratories H-3300-250 Cisplatin 1mg/ml concentrate solution for infusion Accord Healthcare, S.L.U Crystal Violet Sigma-Aldrich 61135 Deferoxamine mesylate salt Sigma-Aldrich D9533 Dimethyl sulfoxide Sigma-Aldrich D26650 DL-Dithiothreitol Sigma-Aldrich D9779 Formaldehyde/Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, 7.4 pH 10 ML Electron Microscopy Sciences 15949 Formalin solution, neutral buffered, 10% Sigma-Aldrich HT501128 Formic acid Merk 5.33002.0050 GeneArtTM Platinum Cas9 Nuclease Thermo Fisher Scientific B25640 Glicerol for molecular biology, R99.0% Sigma-Aldrich G5516 Guanidine hydrochloride Sigma-Aldrich G4505 HaltTM Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific 78440 (Continued on next page) e1 iScience 28, 111758, February 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology Sigma-Aldrich H1758 jetPRIME transfection reagent PolyPlus Transfection 114-01 Lead citrate Electron Microscopy Sciences 22410 Magnesium chloride anhydrous Sigma-Aldrich M8266 Methanol Merck 34860-1L-R MTT (3-(4,5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) ThermoFisher Scientific M6494 Neuraminidase from Clostridium perfringens Sigma-Aldrich N5631 NONIDETTM P 40 Substitute Bioextra, mix Sigma-Aldrich 74385 Osmium tetroxide Electron Microscopy Sciences 20816-12-0 Oxaloacetic acid Sigma-Aldrich 328-42-7 Paraformaldehyde Sigma-Aldrich 158127 PNGase F Glycosidase from Elizabethkingia miricola Promega PROMV4831 Ponceau S anionic dye Sigma-Aldrich P3504 Potassium hydroxide Sigma-Aldrich 306568 Puromycin, Dihydrochloride EMD Millipore 540222 SeaPlaque Agarose Lonza 50101 Sodium borohydride Honeywell FlukaTM 7132 Sodium chloride Sigma-Aldrich 7647-14-5 TritonTM-X Sigma-Aldrich T8787 Trizma base Sigma-Aldrich T6066 Trypan Blue Stain (0.4%) Gibco 15250-061 UAR-EMS Uranyl Acetate Replacement Stain Electron Microscopy Science 22405 Biotinylated Peanut Agglutinin (PNA) Vector Laboratories B-1075-5 Biotinylated Vicia Villosa Lectin (VVA) Vector Laboratories B-1235 Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Staining:Article Title: Multilevel plasticity and altered glycosylation drive aggressiveness in hypoxic and glucose-deprived bladder cancer cells. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] Abcam ab80039 Anti-B3GNT6 polyclonal antibody Invitrogen PA5-90159 Anti-C1GALT1 antibody Novus Biologicals NBP1-88477 Anti-C1GALT1C1 antibody Sigma-Aldrich HPA015632 Anti-GCNT1 antibody Abcam ab102665 Anti-ST3Gal I antibody Abcam ab96129 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific A-11012 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Invitrogen 65-6120 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific 31460 Monoclonal Mouse Anti-Human Ki-67 [MIB-1] Agilent M724029-2 Mouse IgG1 [MOPC-21] isotype control Abcam ab18443 Mouse monoclonal [CA19-9-203] to CA19-9 Abcam ab116024 Mouse monoclonal anti-tag-72 antibody [B72.3 + CC49] Abcam ab199002 Peroxidase AffiniPure Goat anti-Mouse IgG (H+L) Jackson ImmunoResearch 115-035-003 Polyclonal rabbit anti-mouse immunoglobulins/FITC DAKO F0313 Purified anti-human Sialyl Lewis X (dimeric), clone FH6 BioLegend 368102 Rabbit monoclonal [EP1215Y] to HIF-1 alpha Abcam ab51608 Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) Abcam ab23875 Chemicals, peptides, and recombinant proteins 0.05% Trypsin-EDTA Gibco 25300-062 4’,6-Diamidino-2-phenylindole dihydrochloride Thermo Fisher Scientific 62247 5,5’-dithiobis-(2-nitrobenzoic acid) Sigma-Aldrich 69-78-3 Accutase - Enzyme Cell Detachment Medium ThermoFisher Scientific 00-4555-56 Acetic acid Sigma-Aldrich A6283 Acetonitrile Merk 14261-2L Acetyl coenzyme A trisodium salt Sigma-Aldrich A2056 Ammonium acetate,BioXtra, R98% Sigma-Aldrich A7330 Antigen Unmasking Solution, Citrate-Based Vector Laboratories H-3300-250 Cisplatin 1mg/ml concentrate solution for infusion Accord Healthcare, S.L.U Crystal Violet Sigma-Aldrich 61135 Deferoxamine mesylate salt Sigma-Aldrich D9533 Dimethyl sulfoxide Sigma-Aldrich D26650 DL-Dithiothreitol Sigma-Aldrich D9779 Formaldehyde/Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, 7.4 pH 10 ML Electron Microscopy Sciences 15949 Formalin solution, neutral buffered, 10% Sigma-Aldrich HT501128 Formic acid Merk 5.33002.0050 GeneArtTM Platinum Cas9 Nuclease Thermo Fisher Scientific B25640 Glicerol for molecular biology, R99.0% Sigma-Aldrich G5516 Guanidine hydrochloride Sigma-Aldrich G4505 HaltTM Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific 78440 (Continued on next page) e1 iScience 28, 111758, February 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology Sigma-Aldrich H1758 jetPRIME transfection reagent PolyPlus Transfection 114-01 Lead citrate Electron Microscopy Sciences 22410 Magnesium chloride anhydrous Sigma-Aldrich M8266 Methanol Merck 34860-1L-R MTT (3-(4,5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) ThermoFisher Scientific M6494 Neuraminidase from Clostridium perfringens Sigma-Aldrich N5631 NONIDETTM P 40 Substitute Bioextra, mix Sigma-Aldrich 74385 Osmium tetroxide Electron Microscopy Sciences 20816-12-0 Oxaloacetic acid Sigma-Aldrich 328-42-7 Paraformaldehyde Sigma-Aldrich 158127 PNGase F Glycosidase from Elizabethkingia miricola Promega PROMV4831 Ponceau S anionic dye Sigma-Aldrich P3504 Potassium hydroxide Sigma-Aldrich 306568 Puromycin, Dihydrochloride EMD Millipore 540222 SeaPlaque Agarose Lonza 50101 Sodium borohydride Honeywell FlukaTM 7132 Sodium chloride Sigma-Aldrich 7647-14-5 TritonTM-X Sigma-Aldrich T8787 Trizma base Sigma-Aldrich T6066 Trypan Blue Stain (0.4%) Gibco 15250-061 UAR-EMS Uranyl Acetate Replacement Stain Electron Microscopy Science 22405 Biotinylated Peanut Agglutinin (PNA) Vector Laboratories B-1075-5 Biotinylated Vicia Villosa Lectin (VVA) Vector Laboratories B-1235 Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Enzyme-linked Immunosorbent Assay:Article Title: Multilevel plasticity and altered glycosylation drive aggressiveness in hypoxic and glucose-deprived bladder cancer cells. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] Abcam ab80039 Anti-B3GNT6 polyclonal antibody Invitrogen PA5-90159 Anti-C1GALT1 antibody Novus Biologicals NBP1-88477 Anti-C1GALT1C1 antibody Sigma-Aldrich HPA015632 Anti-GCNT1 antibody Abcam ab102665 Anti-ST3Gal I antibody Abcam ab96129 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific A-11012 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Invitrogen 65-6120 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific 31460 Monoclonal Mouse Anti-Human Ki-67 [MIB-1] Agilent M724029-2 Mouse IgG1 [MOPC-21] isotype control Abcam ab18443 Mouse monoclonal [CA19-9-203] to CA19-9 Abcam ab116024 Mouse monoclonal anti-tag-72 antibody [B72.3 + CC49] Abcam ab199002 Peroxidase AffiniPure Goat anti-Mouse IgG (H+L) Jackson ImmunoResearch 115-035-003 Polyclonal rabbit anti-mouse immunoglobulins/FITC DAKO F0313 Purified anti-human Sialyl Lewis X (dimeric), clone FH6 BioLegend 368102 Rabbit monoclonal [EP1215Y] to HIF-1 alpha Abcam ab51608 Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) Abcam ab23875 Chemicals, peptides, and recombinant proteins 0.05% Trypsin-EDTA Gibco 25300-062 4’,6-Diamidino-2-phenylindole dihydrochloride Thermo Fisher Scientific 62247 5,5’-dithiobis-(2-nitrobenzoic acid) Sigma-Aldrich 69-78-3 Accutase - Enzyme Cell Detachment Medium ThermoFisher Scientific 00-4555-56 Acetic acid Sigma-Aldrich A6283 Acetonitrile Merk 14261-2L Acetyl coenzyme A trisodium salt Sigma-Aldrich A2056 Ammonium acetate,BioXtra, R98% Sigma-Aldrich A7330 Antigen Unmasking Solution, Citrate-Based Vector Laboratories H-3300-250 Cisplatin 1mg/ml concentrate solution for infusion Accord Healthcare, S.L.U Crystal Violet Sigma-Aldrich 61135 Deferoxamine mesylate salt Sigma-Aldrich D9533 Dimethyl sulfoxide Sigma-Aldrich D26650 DL-Dithiothreitol Sigma-Aldrich D9779 Formaldehyde/Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, 7.4 pH 10 ML Electron Microscopy Sciences 15949 Formalin solution, neutral buffered, 10% Sigma-Aldrich HT501128 Formic acid Merk 5.33002.0050 GeneArtTM Platinum Cas9 Nuclease Thermo Fisher Scientific B25640 Glicerol for molecular biology, R99.0% Sigma-Aldrich G5516 Guanidine hydrochloride Sigma-Aldrich G4505 HaltTM Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific 78440 (Continued on next page) e1 iScience 28, 111758, February 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology Sigma-Aldrich H1758 jetPRIME transfection reagent PolyPlus Transfection 114-01 Lead citrate Electron Microscopy Sciences 22410 Magnesium chloride anhydrous Sigma-Aldrich M8266 Methanol Merck 34860-1L-R MTT (3-(4,5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) ThermoFisher Scientific M6494 Neuraminidase from Clostridium perfringens Sigma-Aldrich N5631 NONIDETTM P 40 Substitute Bioextra, mix Sigma-Aldrich 74385 Osmium tetroxide Electron Microscopy Sciences 20816-12-0 Oxaloacetic acid Sigma-Aldrich 328-42-7 Paraformaldehyde Sigma-Aldrich 158127 PNGase F Glycosidase from Elizabethkingia miricola Promega PROMV4831 Ponceau S anionic dye Sigma-Aldrich P3504 Potassium hydroxide Sigma-Aldrich 306568 Puromycin, Dihydrochloride EMD Millipore 540222 SeaPlaque Agarose Lonza 50101 Sodium borohydride Honeywell FlukaTM 7132 Sodium chloride Sigma-Aldrich 7647-14-5 TritonTM-X Sigma-Aldrich T8787 Trizma base Sigma-Aldrich T6066 Trypan Blue Stain (0.4%) Gibco 15250-061 UAR-EMS Uranyl Acetate Replacement Stain Electron Microscopy Science 22405 Biotinylated Peanut Agglutinin (PNA) Vector Laboratories B-1075-5 Biotinylated Vicia Villosa Lectin (VVA) Vector Laboratories B-1235 Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Flow Cytometry:Article Title: Multilevel plasticity and altered glycosylation drive aggressiveness in hypoxic and glucose-deprived bladder cancer cells. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] Abcam ab80039 Anti-B3GNT6 polyclonal antibody Invitrogen PA5-90159 Anti-C1GALT1 antibody Novus Biologicals NBP1-88477 Anti-C1GALT1C1 antibody Sigma-Aldrich HPA015632 Anti-GCNT1 antibody Abcam ab102665 Anti-ST3Gal I antibody Abcam ab96129 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific A-11012 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Invitrogen 65-6120 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific 31460 Monoclonal Mouse Anti-Human Ki-67 [MIB-1] Agilent M724029-2 Mouse IgG1 [MOPC-21] isotype control Abcam ab18443 Mouse monoclonal [CA19-9-203] to CA19-9 Abcam ab116024 Mouse monoclonal anti-tag-72 antibody [B72.3 + CC49] Abcam ab199002 Peroxidase AffiniPure Goat anti-Mouse IgG (H+L) Jackson ImmunoResearch 115-035-003 Polyclonal rabbit anti-mouse immunoglobulins/FITC DAKO F0313 Purified anti-human Sialyl Lewis X (dimeric), clone FH6 BioLegend 368102 Rabbit monoclonal [EP1215Y] to HIF-1 alpha Abcam ab51608 Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) Abcam ab23875 Chemicals, peptides, and recombinant proteins 0.05% Trypsin-EDTA Gibco 25300-062 4’,6-Diamidino-2-phenylindole dihydrochloride Thermo Fisher Scientific 62247 5,5’-dithiobis-(2-nitrobenzoic acid) Sigma-Aldrich 69-78-3 Accutase - Enzyme Cell Detachment Medium ThermoFisher Scientific 00-4555-56 Acetic acid Sigma-Aldrich A6283 Acetonitrile Merk 14261-2L Acetyl coenzyme A trisodium salt Sigma-Aldrich A2056 Ammonium acetate,BioXtra, R98% Sigma-Aldrich A7330 Antigen Unmasking Solution, Citrate-Based Vector Laboratories H-3300-250 Cisplatin 1mg/ml concentrate solution for infusion Accord Healthcare, S.L.U Crystal Violet Sigma-Aldrich 61135 Deferoxamine mesylate salt Sigma-Aldrich D9533 Dimethyl sulfoxide Sigma-Aldrich D26650 DL-Dithiothreitol Sigma-Aldrich D9779 Formaldehyde/Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, 7.4 pH 10 ML Electron Microscopy Sciences 15949 Formalin solution, neutral buffered, 10% Sigma-Aldrich HT501128 Formic acid Merk 5.33002.0050 GeneArtTM Platinum Cas9 Nuclease Thermo Fisher Scientific B25640 Glicerol for molecular biology, R99.0% Sigma-Aldrich G5516 Guanidine hydrochloride Sigma-Aldrich G4505 HaltTM Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific 78440 (Continued on next page) e1 iScience 28, 111758, February 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology Sigma-Aldrich H1758 jetPRIME transfection reagent PolyPlus Transfection 114-01 Lead citrate Electron Microscopy Sciences 22410 Magnesium chloride anhydrous Sigma-Aldrich M8266 Methanol Merck 34860-1L-R MTT (3-(4,5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) ThermoFisher Scientific M6494 Neuraminidase from Clostridium perfringens Sigma-Aldrich N5631 NONIDETTM P 40 Substitute Bioextra, mix Sigma-Aldrich 74385 Osmium tetroxide Electron Microscopy Sciences 20816-12-0 Oxaloacetic acid Sigma-Aldrich 328-42-7 Paraformaldehyde Sigma-Aldrich 158127 PNGase F Glycosidase from Elizabethkingia miricola Promega PROMV4831 Ponceau S anionic dye Sigma-Aldrich P3504 Potassium hydroxide Sigma-Aldrich 306568 Puromycin, Dihydrochloride EMD Millipore 540222 SeaPlaque Agarose Lonza 50101 Sodium borohydride Honeywell FlukaTM 7132 Sodium chloride Sigma-Aldrich 7647-14-5 TritonTM-X Sigma-Aldrich T8787 Trizma base Sigma-Aldrich T6066 Trypan Blue Stain (0.4%) Gibco 15250-061 UAR-EMS Uranyl Acetate Replacement Stain Electron Microscopy Science 22405 Biotinylated Peanut Agglutinin (PNA) Vector Laboratories B-1075-5 Biotinylated Vicia Villosa Lectin (VVA) Vector Laboratories B-1235 Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), ATP Assay:Article Title: Multilevel plasticity and altered glycosylation drive aggressiveness in hypoxic and glucose-deprived bladder cancer cells. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] Abcam ab80039 Anti-B3GNT6 polyclonal antibody Invitrogen PA5-90159 Anti-C1GALT1 antibody Novus Biologicals NBP1-88477 Anti-C1GALT1C1 antibody Sigma-Aldrich HPA015632 Anti-GCNT1 antibody Abcam ab102665 Anti-ST3Gal I antibody Abcam ab96129 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific A-11012 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Invitrogen 65-6120 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific 31460 Monoclonal Mouse Anti-Human Ki-67 [MIB-1] Agilent M724029-2 Mouse IgG1 [MOPC-21] isotype control Abcam ab18443 Mouse monoclonal [CA19-9-203] to CA19-9 Abcam ab116024 Mouse monoclonal anti-tag-72 antibody [B72.3 + CC49] Abcam ab199002 Peroxidase AffiniPure Goat anti-Mouse IgG (H+L) Jackson ImmunoResearch 115-035-003 Polyclonal rabbit anti-mouse immunoglobulins/FITC DAKO F0313 Purified anti-human Sialyl Lewis X (dimeric), clone FH6 BioLegend 368102 Rabbit monoclonal [EP1215Y] to HIF-1 alpha Abcam ab51608 Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) Abcam ab23875 Chemicals, peptides, and recombinant proteins 0.05% Trypsin-EDTA Gibco 25300-062 4’,6-Diamidino-2-phenylindole dihydrochloride Thermo Fisher Scientific 62247 5,5’-dithiobis-(2-nitrobenzoic acid) Sigma-Aldrich 69-78-3 Accutase - Enzyme Cell Detachment Medium ThermoFisher Scientific 00-4555-56 Acetic acid Sigma-Aldrich A6283 Acetonitrile Merk 14261-2L Acetyl coenzyme A trisodium salt Sigma-Aldrich A2056 Ammonium acetate,BioXtra, R98% Sigma-Aldrich A7330 Antigen Unmasking Solution, Citrate-Based Vector Laboratories H-3300-250 Cisplatin 1mg/ml concentrate solution for infusion Accord Healthcare, S.L.U Crystal Violet Sigma-Aldrich 61135 Deferoxamine mesylate salt Sigma-Aldrich D9533 Dimethyl sulfoxide Sigma-Aldrich D26650 DL-Dithiothreitol Sigma-Aldrich D9779 Formaldehyde/Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, 7.4 pH 10 ML Electron Microscopy Sciences 15949 Formalin solution, neutral buffered, 10% Sigma-Aldrich HT501128 Formic acid Merk 5.33002.0050 GeneArtTM Platinum Cas9 Nuclease Thermo Fisher Scientific B25640 Glicerol for molecular biology, R99.0% Sigma-Aldrich G5516 Guanidine hydrochloride Sigma-Aldrich G4505 HaltTM Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific 78440 (Continued on next page) e1 iScience 28, 111758, February 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology Sigma-Aldrich H1758 jetPRIME transfection reagent PolyPlus Transfection 114-01 Lead citrate Electron Microscopy Sciences 22410 Magnesium chloride anhydrous Sigma-Aldrich M8266 Methanol Merck 34860-1L-R MTT (3-(4,5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) ThermoFisher Scientific M6494 Neuraminidase from Clostridium perfringens Sigma-Aldrich N5631 NONIDETTM P 40 Substitute Bioextra, mix Sigma-Aldrich 74385 Osmium tetroxide Electron Microscopy Sciences 20816-12-0 Oxaloacetic acid Sigma-Aldrich 328-42-7 Paraformaldehyde Sigma-Aldrich 158127 PNGase F Glycosidase from Elizabethkingia miricola Promega PROMV4831 Ponceau S anionic dye Sigma-Aldrich P3504 Potassium hydroxide Sigma-Aldrich 306568 Puromycin, Dihydrochloride EMD Millipore 540222 SeaPlaque Agarose Lonza 50101 Sodium borohydride Honeywell FlukaTM 7132 Sodium chloride Sigma-Aldrich 7647-14-5 TritonTM-X Sigma-Aldrich T8787 Trizma base Sigma-Aldrich T6066 Trypan Blue Stain (0.4%) Gibco 15250-061 UAR-EMS Uranyl Acetate Replacement Stain Electron Microscopy Science 22405 Biotinylated Peanut Agglutinin (PNA) Vector Laboratories B-1075-5 Biotinylated Vicia Villosa Lectin (VVA) Vector Laboratories B-1235 Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Phospho-proteomics:Article Title: Multilevel plasticity and altered glycosylation drive aggressiveness in hypoxic and glucose-deprived bladder cancer cells. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] Abcam ab80039 Anti-B3GNT6 polyclonal antibody Invitrogen PA5-90159 Anti-C1GALT1 antibody Novus Biologicals NBP1-88477 Anti-C1GALT1C1 antibody Sigma-Aldrich HPA015632 Anti-GCNT1 antibody Abcam ab102665 Anti-ST3Gal I antibody Abcam ab96129 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific A-11012 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Invitrogen 65-6120 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific 31460 Monoclonal Mouse Anti-Human Ki-67 [MIB-1] Agilent M724029-2 Mouse IgG1 [MOPC-21] isotype control Abcam ab18443 Mouse monoclonal [CA19-9-203] to CA19-9 Abcam ab116024 Mouse monoclonal anti-tag-72 antibody [B72.3 + CC49] Abcam ab199002 Peroxidase AffiniPure Goat anti-Mouse IgG (H+L) Jackson ImmunoResearch 115-035-003 Polyclonal rabbit anti-mouse immunoglobulins/FITC DAKO F0313 Purified anti-human Sialyl Lewis X (dimeric), clone FH6 BioLegend 368102 Rabbit monoclonal [EP1215Y] to HIF-1 alpha Abcam ab51608 Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) Abcam ab23875 Chemicals, peptides, and recombinant proteins 0.05% Trypsin-EDTA Gibco 25300-062 4’,6-Diamidino-2-phenylindole dihydrochloride Thermo Fisher Scientific 62247 5,5’-dithiobis-(2-nitrobenzoic acid) Sigma-Aldrich 69-78-3 Accutase - Enzyme Cell Detachment Medium ThermoFisher Scientific 00-4555-56 Acetic acid Sigma-Aldrich A6283 Acetonitrile Merk 14261-2L Acetyl coenzyme A trisodium salt Sigma-Aldrich A2056 Ammonium acetate,BioXtra, R98% Sigma-Aldrich A7330 Antigen Unmasking Solution, Citrate-Based Vector Laboratories H-3300-250 Cisplatin 1mg/ml concentrate solution for infusion Accord Healthcare, S.L.U Crystal Violet Sigma-Aldrich 61135 Deferoxamine mesylate salt Sigma-Aldrich D9533 Dimethyl sulfoxide Sigma-Aldrich D26650 DL-Dithiothreitol Sigma-Aldrich D9779 Formaldehyde/Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, 7.4 pH 10 ML Electron Microscopy Sciences 15949 Formalin solution, neutral buffered, 10% Sigma-Aldrich HT501128 Formic acid Merk 5.33002.0050 GeneArtTM Platinum Cas9 Nuclease Thermo Fisher Scientific B25640 Glicerol for molecular biology, R99.0% Sigma-Aldrich G5516 Guanidine hydrochloride Sigma-Aldrich G4505 HaltTM Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific 78440 (Continued on next page) e1 iScience 28, 111758, February 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology Sigma-Aldrich H1758 jetPRIME transfection reagent PolyPlus Transfection 114-01 Lead citrate Electron Microscopy Sciences 22410 Magnesium chloride anhydrous Sigma-Aldrich M8266 Methanol Merck 34860-1L-R MTT (3-(4,5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) ThermoFisher Scientific M6494 Neuraminidase from Clostridium perfringens Sigma-Aldrich N5631 NONIDETTM P 40 Substitute Bioextra, mix Sigma-Aldrich 74385 Osmium tetroxide Electron Microscopy Sciences 20816-12-0 Oxaloacetic acid Sigma-Aldrich 328-42-7 Paraformaldehyde Sigma-Aldrich 158127 PNGase F Glycosidase from Elizabethkingia miricola Promega PROMV4831 Ponceau S anionic dye Sigma-Aldrich P3504 Potassium hydroxide Sigma-Aldrich 306568 Puromycin, Dihydrochloride EMD Millipore 540222 SeaPlaque Agarose Lonza 50101 Sodium borohydride Honeywell FlukaTM 7132 Sodium chloride Sigma-Aldrich 7647-14-5 TritonTM-X Sigma-Aldrich T8787 Trizma base Sigma-Aldrich T6066 Trypan Blue Stain (0.4%) Gibco 15250-061 UAR-EMS Uranyl Acetate Replacement Stain Electron Microscopy Science 22405 Biotinylated Peanut Agglutinin (PNA) Vector Laboratories B-1075-5 Biotinylated Vicia Villosa Lectin (VVA) Vector Laboratories B-1235 Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Transcriptomics:Article Title: Multilevel plasticity and altered glycosylation drive aggressiveness in hypoxic and glucose-deprived bladder cancer cells. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2] Abcam ab80039 Anti-B3GNT6 polyclonal antibody Invitrogen PA5-90159 Anti-C1GALT1 antibody Novus Biologicals NBP1-88477 Anti-C1GALT1C1 antibody Sigma-Aldrich HPA015632 Anti-GCNT1 antibody Abcam ab102665 Anti-ST3Gal I antibody Abcam ab96129 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific A-11012 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Invitrogen 65-6120 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific 31460 Monoclonal Mouse Anti-Human Ki-67 [MIB-1] Agilent M724029-2 Mouse IgG1 [MOPC-21] isotype control Abcam ab18443 Mouse monoclonal [CA19-9-203] to CA19-9 Abcam ab116024 Mouse monoclonal anti-tag-72 antibody [B72.3 + CC49] Abcam ab199002 Peroxidase AffiniPure Goat anti-Mouse IgG (H+L) Jackson ImmunoResearch 115-035-003 Polyclonal rabbit anti-mouse immunoglobulins/FITC DAKO F0313 Purified anti-human Sialyl Lewis X (dimeric), clone FH6 BioLegend 368102 Rabbit monoclonal [EP1215Y] to HIF-1 alpha Abcam ab51608 Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) Abcam ab23875 Chemicals, peptides, and recombinant proteins 0.05% Trypsin-EDTA Gibco 25300-062 4’,6-Diamidino-2-phenylindole dihydrochloride Thermo Fisher Scientific 62247 5,5’-dithiobis-(2-nitrobenzoic acid) Sigma-Aldrich 69-78-3 Accutase - Enzyme Cell Detachment Medium ThermoFisher Scientific 00-4555-56 Acetic acid Sigma-Aldrich A6283 Acetonitrile Merk 14261-2L Acetyl coenzyme A trisodium salt Sigma-Aldrich A2056 Ammonium acetate,BioXtra, R98% Sigma-Aldrich A7330 Antigen Unmasking Solution, Citrate-Based Vector Laboratories H-3300-250 Cisplatin 1mg/ml concentrate solution for infusion Accord Healthcare, S.L.U Crystal Violet Sigma-Aldrich 61135 Deferoxamine mesylate salt Sigma-Aldrich D9533 Dimethyl sulfoxide Sigma-Aldrich D26650 DL-Dithiothreitol Sigma-Aldrich D9779 Formaldehyde/Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, 7.4 pH 10 ML Electron Microscopy Sciences 15949 Formalin solution, neutral buffered, 10% Sigma-Aldrich HT501128 Formic acid Merk 5.33002.0050 GeneArtTM Platinum Cas9 Nuclease Thermo Fisher Scientific B25640 Glicerol for molecular biology, R99.0% Sigma-Aldrich G5516 Guanidine hydrochloride Sigma-Aldrich G4505 HaltTM Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific 78440 (Continued on next page) e1 iScience 28, 111758, February 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology Sigma-Aldrich H1758 jetPRIME transfection reagent PolyPlus Transfection 114-01 Lead citrate Electron Microscopy Sciences 22410 Magnesium chloride anhydrous Sigma-Aldrich M8266 Methanol Merck 34860-1L-R MTT (3-(4,5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) ThermoFisher Scientific M6494 Neuraminidase from Clostridium perfringens Sigma-Aldrich N5631 NONIDETTM P 40 Substitute Bioextra, mix Sigma-Aldrich 74385 Osmium tetroxide Electron Microscopy Sciences 20816-12-0 Oxaloacetic acid Sigma-Aldrich 328-42-7 Paraformaldehyde Sigma-Aldrich 158127 PNGase F Glycosidase from Elizabethkingia miricola Promega PROMV4831 Ponceau S anionic dye Sigma-Aldrich P3504 Potassium hydroxide Sigma-Aldrich 306568 Puromycin, Dihydrochloride EMD Millipore 540222 SeaPlaque Agarose Lonza 50101 Sodium borohydride Honeywell FlukaTM 7132 Sodium chloride Sigma-Aldrich 7647-14-5 TritonTM-X Sigma-Aldrich T8787 Trizma base Sigma-Aldrich T6066 Trypan Blue Stain (0.4%) Gibco 15250-061 UAR-EMS Uranyl Acetate Replacement Stain Electron Microscopy Science 22405 Biotinylated Peanut Agglutinin (PNA) Vector Laboratories B-1075-5 Biotinylated Vicia Villosa Lectin (VVA) Vector Laboratories B-1235 Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), other:Article Title: ARID1A mutation drives gastric tumorigenesis via activating type 2 immune dominant microenvironment Article Snippet: Saline:Article Title: Lectin binding to pectoral fin of neonate little skates reared under ambient and projected-end-of-century temperature regimes. Article Snippet: Funding information National Institutes of Health (NIH), Grant/Award Number: R15DK106688 Abstract The glycosylation of macromolecules can vary both among tissue structural components and by adverse conditions, potentially providing an alternative marker of stress in organisms.. Lectins are proteins that bind carbohydrate moieties and lectin histochemistry is a common method to visualize microstructures in biological specimens and diagnose pathophysiological states in human tissues known to alter glycan profiles.. However, this technique is not commonly used to assess broad‐ spectrum changes in cellular glycosylation in response to environmental stressors. Incubation:Article Title: Lectin binding to pectoral fin of neonate little skates reared under ambient and projected-end-of-century temperature regimes. Article Snippet: Funding information National Institutes of Health (NIH), Grant/Award Number: R15DK106688 Abstract The glycosylation of macromolecules can vary both among tissue structural components and by adverse conditions, potentially providing an alternative marker of stress in organisms.. Lectins are proteins that bind carbohydrate moieties and lectin histochemistry is a common method to visualize microstructures in biological specimens and diagnose pathophysiological states in human tissues known to alter glycan profiles.. However, this technique is not commonly used to assess broad‐ spectrum changes in cellular glycosylation in response to environmental stressors. Whole Genome Amplification:Article Title: Lectin binding to pectoral fin of neonate little skates reared under ambient and projected-end-of-century temperature regimes. Article Snippet: Funding information National Institutes of Health (NIH), Grant/Award Number: R15DK106688 Abstract The glycosylation of macromolecules can vary both among tissue structural components and by adverse conditions, potentially providing an alternative marker of stress in organisms.. Lectins are proteins that bind carbohydrate moieties and lectin histochemistry is a common method to visualize microstructures in biological specimens and diagnose pathophysiological states in human tissues known to alter glycan profiles.. However, this technique is not commonly used to assess broad‐ spectrum changes in cellular glycosylation in response to environmental stressors. |
![Hypoxia and low glucose impair O -glycans extension in BLCA, originating a simple cancer cell glycophenotype (A) BLCA cells exposed to hypoxia and low glucose exhibit less abundant, simpler, and shorter glycomes, lacking extensions beyond core 1 structures. nanoLC-MS/MS analysis shows that this glycophenotype is characterized by sialylated T antigens and core 3, likely due to decreased typical core 1 and 2 structures. DFX-treated cells, stabilizing HIF-1α, show no significant alterations in the glycome, suggesting that changes observed in stressed cells are not driven by HIF-1α. (B) <t>Lectin</t> affinity studies show significant upregulation of Tn and sialylated T antigens (recognized by PNA lectin after Neuraminidase [NeuAse] digestion) under stress, in accordance with MS-based glycomics. Notably, core 3 O -glycans (evaluated by GSL II lectin after PNGase F digestion) remain unchanged, highlighting that cellular stress primarily suppresses core 1/2 O -glycans, rather than increasing core 3 O -glycans. (C) Glucose suppression is the primary driver of glycome remodeling, which can be reversed by reoxygenation and restoration of glucose. (D) Glycogene remodeling is primarily driven by the combined effects of hypoxia and glucose deprivation and leads to a premature halt in glycans extension beyond core 1. C1GALT1C1 , necessary for core 1 biosynthesis, is downregulated, whereas ST3GAL1 , 3 , and 4 are overexpressed, increasing sialylated T antigens and inhibiting core 2 formation. Downregulation of GCNT4 also contributes to core 2 inhibition. Interestingly, elevated GCNT1 and GCNT3 potentially counterbalance core 2 suppression. (E) Quantification of key enzymes involved in O -glycan elongation (C1GalT1; Cosmc; BGnT-6; C2GNT; ST3Gal-I) shows significant upregulation of ST3Gal-1 in stressed cells, consistent with transcriptomics. The others remain unchanged, indicating distinct regulation between glycogenes and glycosyltransferases under these conditions. Bold circles and triangles represent statistically significant changes in T24 and 5637 cell lines, respectively. Error bars represent mean ± SD for three independent experiments. Mann-Whitney Test was used for statistical analysis. Results were considered statistically significant when p < 0.05.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1300/pmc11791300/pmc11791300__gr5.jpg)
